Influence of botulinum toxin A in pain perception and condyle-fossa relationship after the management of temporomandibular dysfunction: a randomized controlled clinical trial.

OBJECTIVES: To investigate the pain perception (PP) and condyle-fossa relationship (CFR) after botulinum toxin A (BoNTA) injection in the masseter muscles of painful muscular temporomandibular dysfunction (TMD) patients. MATERIALS AND METHODS: Fourteen women (aged 29.7 ± 5.4 years) diagnosed with myogenic TMD were randomized in the BoNTA-treated group (TG) and control group (CG). TG masseter muscles (n = 7) were bilaterally injected with 30 U. The CG (n = 7) were injected with saline injections. Condyle-fossa relationship (CFR) spaces were measured in sagittal (SP) and frontal planes (FP) of images of cone-beam computed tomography (CBCT) done before (T0) and after 30 days' interventions (T1). Visual analogue scale (VAS) measured the patients' TMD pain perception (PP). Data were compared by generalized linear models considering the results over time (α = .05). RESULTS: There were no statistical differences in CFR in the SP or FP for TG and CG over time (p ˃ .05), except for frontal lateral space CFR (p < .05). In both groups, the condyle was positioned medially after interventions. Frontal lateral space increased in TG for both, left and right sides, over time (p < .05), as well as PP decreased over time (p < .05) for TG and CG. CONCLUSIONS: The results depicted that there was no significant association with BoNTA injection in TMD masseter muscles in PP and CFR, except considering the frontal lateral space of CFR. CLINICAL RELEVANCE: BoNTA injection in the masseter muscles may not promote clinically significant shifts in the condyle-fossa relationships of muscular TMD patients.

The effects of miniscrew-assisted rapid palatal expansion on the upper airway of adults with midpalatal suture in the last two degrees of ossification.

BACKGROUND: Transverse deficiencies of the maxillary basal bone have been treated in adult patients using miniscrew-assisted rapid palatal expansion (MARPE) therapy. However, the midpalatal suture's degree of ossification may affect the upper airway dimensions. This study compared the volumetric changes of the total upper, retropalatal, retroglossal airways, and the minimal transverse airway constriction after MARPE therapy in patients with midpalatal suture in the last stages of ossification. METHODS: This controlled clinical trial included a total of 20 adult patients (mean age 24.5 ± 6.2 years) with maxillary atresia treated with MARPE. Preoperative (T0) cone-beam computed tomography scans were used to determine the degree of midpalatal suture ossification. Two groups were formed considering the last two stages of sutural ossification "D" or "E" (n = 10 per group). After 120 days of the therapy (T1), cone-beam computed tomography assessments were performed to compare the pre and post-treatment outcomes. The total upper, retropalatal, and retroglossal airways and the minimal transverse airway constriction were evaluated. The three-dimensional reconstruction was performed with OsiriX MD software. The comparisons were carried out using mixed models for repeated measures at fixed time points (α = 0.05). RESULTS: Groups D and E showed no significant difference for any of the analyzed parameters (P > 0.05). Both groups showed a statistically significant increase for all airway segments after the treatment with MARPE (P < 0.05). The total upper airway increased (11.6% and 16.1%) for groups D and E, respectively (P = 0.3356). CONCLUSIONS: MARPE therapy resulted in dimensional gains of the upper airway for adult patients, irrespective of the intermaxillary sutural degree of ossification.

Influence of NaHCO3 powder on color and roughness of the composite submitted to bleaching and euterpe oleracea / Influencia del polvo de NaHCO 3 en el color y rugosidad del composite sometido a blanqueo y euterpe oleracea

Aim: This study aims to evaluate the surface roughness and susceptibility to staining of bleached composite resin with 22% carbamide peroxide, as well as the effect of subsequent prophylaxis with NaHCO3 powder. Material and Methods: Forty disk-shaped (2 × 6 mm) specimens of composite resin (Z250 XT) were prepared. Half of the specimens were subjected to bleaching with 22% carbamide peroxide, and the other half were stored in artificial saliva. In sequence, all specimens were immersed in acai juice (Euterpe oleracea) for 4 h for 14 days, and subdivided into two groups. Group 1 samples were subjected to prophylaxis treatment, while group 2 samples were subjected to treatment with artificial saliva. Surface roughness (Ra) and color (ΔE*) were measured after polishing (T0), bleaching (T1), immersion in acai juice (T2), and application of NaHCO3 powder (T3) using a profilometer and a spectrophotometer. Results: Statistical analyses (analysis of variance and Tukey's test, p≤0.05) revealed that regarding color there was statistically significance for the factors in isolation, except for the factor bleaching. For both color and surface roughness there was statistically significant difference for the interaction, except for the interaction between NaHCO3 power and bleaching. Conclusion: The NaHCO3 air-powder polishing decreases the staining of the composite resin; however, it increases the surface roughness. With respect to the color variable, the whitening factor had no significant effect on the tested material; however, it increases surface roughness.

Objetivo: Este estudio tiene como objetivo evaluar la rugosidad de la superficie y la susceptibilidad a la tinción de la resina compuesta blanqueada con peróxido de carbamida al 22%, así como el efecto de la profilaxis posterior con polvo de NaHCO3. Material y Métodos: Se prepararon cuarenta especímenes en forma de disco (2 × 6 mm) de resina compuesta (Z250 XT). La mitad de los especímenes se sometieron a blanqueo con peróxido de carbamida al 22% y la otra mitad se almacenó en saliva artificial. En secuencia, todos los especímenes se sumergieron en jugo de acai (Euterpe oleracea) durante 4h durante 14 días, y se subdividieron en dos grupos. Las muestras del grupo 1 se sometieron a tratamiento profiláctico, mientras que las muestras del grupo 2 se sometieron a tratamiento con saliva artificial. La rugosidad de la superficie (Ra) y el color (ΔE*) se midieron después del pulido (T0), el blanqueo (T1), la inmersión en jugo de acai (T2) y la aplicación de polvo de NaHCO3 (T3) utilizando un perfilómetro y un espectrofotómetro. Resultados: Los análisis estadísticos (análisis de varianza y prueba de Tukey, p≤0.05) revelaron que en relación al color hubo significancia estadística para los factores en forma aislada, excepto para el factor blanqueamiento. Tanto para el color como para la rugosidad de la superficie hubo una diferencia estadísticamente significativa para la interacción, excepto para la interacción entre el poder de NaHCO3 y el blanqueo. Conclusión: El pulido al aire con polvo de NaHCO3 disminuye el manchado de la resina compuesta; sin embargo, aumenta la rugosidad de la superficie. Con respecto a la variable color, el factor de blanqueamiento no tuvo efecto significativo sobre el material ensayado; sin embargo, aumenta la rugosidad de la superficie.

The impact of erosive tooth wear related to masticatory quality in an indigenous Brazilian population: A cross-sectional study.

OBJECTIVE: The study aimed to associate the impact of tooth wear with the perception of masticatory quality and eating habits in a Brazilian indigenous population. METHODS: A cross-sectional observational study was carried out with 197 indigenous from the Macuxi tribe (Roraima, Brazil) divided into age groups (adolescents and adults). Tooth wear was determined by the Basic Erosive Wear Examination (BEWE). The masticatory quality was evaluated using the Quality of Masticatory Function Questionnaire (QMFQ). Study participants also answered questions regarding eating habits and frequency of food consumption. Logistic regression models and crude Odds Ratios were estimated with 95% confidence intervals. Variables with P<0.20 in simple analyses were tested in a multiple logistic regression model; those with P ≤ 0.05 remained in the model. RESULTS: Tooth wear was observed in 38.1% of the indigenous, and 48.2% of them reported having their masticatory function affected; banana was the most frequently consumed item (54.3%); there was a significant association between adulthood and the presence of tooth wear (P<0.0001). CONCLUSIONS: Tooth wear related to eating habits and poor masticatory quality did not impact the oral health condition of the studied indigenous population. However, adulthood was associated with tooth wear in this sample.

Dentoskeletal changes and their correlations after micro-implant-assisted palatal expansion (MARPE) in adults with advanced midpalatal suture ossification.

OBJECTIVES: To evaluate and correlate the dentoskeletal changes of adult patients after miniscrew-assisted rapid palatal expander (MARPE) in the two final stages of midpalatal suture ossification. MATERIALS AND METHODS: This interventional pre-post clinical study consisted of 20 adults (24.9±1.8 years), with transverse maxillary atresia, divided into two subgroups (n=10) based on the ossification degree of the midpalatal suture: stage D, fusion completed in the palatine bone; and stage E, fusion anteriorly in the maxilla. Cone beam computed tomography (CBCT) in the pre- (T0) and post-treatment (T1) time intervals were used to evaluate the anterior and posterior sutural opening, activation of the expander screw, vestibular-lingual inclinations, and widths of first premolars and first molars. Maxillary interincisor diastema was measured on patients' plaster models in the same timepoints. The Wilcoxon-Mann-Whitney test, generalized linear models (GLM), and chi-square (χ2) test were employed. RESULTS: All variables significantly increased after MARPE therapy regardless of the ossification degree (P<0.05). Patients with stage D suture presented wider interincisal diastema and maxillary suture opening (on both regions, anterior and posterior) when compared with patients with stage E (P<0.05). Maxillary interincisor diastema was positively associated with anterior and posterior suture opening for all subjects (P<0.05). MARPE therapy was unsuccessful in six patients, mostly presented by individuals with E maturation stage of the midpalatal suture. CONCLUSIONS: After MARPE, a significant correction in the transverse defect of the maxillary basal bone was achieved for adults in the last two midpalatal suture maturation stages. CLINICAL RELEVANCE: Adult patients in stage D of maxillary suture ossification are more susceptible to dentoskeletal changes following MARPE therapy as compared to patients with stage E.

Use of dentifrices and mouthwashes during orthodontic treatment in the clinical management of orthodontists / Utilização de dentifrícios e colutórios durante o tratamento ortodôntico na conduta clínica dos ortodontistas

ABSTRACT Objective: To evaluate the knowledge of orthodontic professionals regarding the prescription of dentifrices and antiseptics during orthodontic treatment. Methods: An observational and descriptive exploratory study of national scope was conducted with 440 Brazilian orthodontists, based on previous sample calculations, to evaluate the clinical practice of orthodontists in prescribing dentifrices and antiseptics. Self-managed electronic questionnaires were sent to orthodontic professionals. The data were analyzed by means of absolute and relative frequency distribution tables. Results: It was verified that the most indicated mouthwash (39.8%) was Colgate Periogard® and 421 of the participants (95.7%) know its active ingredient (chlorhexidine digluconate). Besides Colgate Periogard® antiseptic, the participants were also questioned about which active ingredient present on the following antiseptics, whose assertive answers were in the following order of %, Cepacol® (Sanofi-Aventis®) with 60.7%, Colgate Plax® (Colgate®) with 54.5% and Listerine® (Jhonson&Jhonson®) with 51.6%. As for the chlorhexidine therapeutic regime, 63.9% have stated assertively its use, being this one of 2 times a day during 7 to 10 days. The most indicated tooth was Colgate Total 12®, and 60.18% of these individuals correctly indicated its active compound. Conclusion: According to the methodology employed, it could be concluded that orthodontic professionals know and prescribe methods of chemical control to patients during orthodontic treatment and most identify the active chemical compounds present in antiseptic and tooth formulas.

RESUMO Objetivo: Avaliar o conhecimento dos profissionais da ortodontia quanto à prescrição de dentifrícios e antissépticos durante o tratamento ortodôntico. Métodos: Foi realizado um estudo observacional e descritivo exploratório de abrangência nacional com 440 ortodontistas brasileiros, baseado em cálculo amostral prévio, para avaliação da prática clínica dos ortodontistas na prescrição de dentifrícios e antissépticos. Questionários eletrônicos auto administrados foram enviados aos profissionais da Ortodontia. Os dados foram analisados por meio de tabelas de distribuição de frequência absolutas e relativas. Resultados: Verificou-se que o antisséptico bucal mais indicado (39,8%) foi o Colgate Periogard® (Colgate®) e 421 dos participantes (95,7%) conhecem o seu ingrediente ativo (digluconato de clorexidina). Além do antisséptico Colgate Periogard®, os participantes também foram questionados sobre qual o ingrediente ativo presente nos seguintes antissépticos, cujas respostas assertivas estavam na seguinte ordem %, Cepacol® (Sanofi-Aventis®) com 60,7%, Colgate Plax® (Colgate®) com 54,5% e Listerine® (Jhonson&Jhonson®) com 51,6%. Quanto ao regime terapêutico da clorexidina 63,9% indicaram assertivamente sua utilização sendo a mesma de 2 vezes ao dia durante 7 a 10 dias. O dentifrício mais indicado foi o Colgate Total 12®, sendo que 60,18% desses indivíduos indicaram corretamente seu composto ativo. Conclusão: De acordo com a metodologia empregada, pôde-se concluir que os profissionais da ortodontia conhecem e prescrevem métodos de controle químico aos pacientes durante o tratamento ortodôntico e a maioria identifica os compostos químicos ativos presentes nas fórmulas de antissépticos e dentifrícios.

Avaliação do tratamento de superfície de resina composta na resistência de união de bráquetes metálicos / Evaluation of composite resin surface treatment in the union resistance of metallic brackets

O objetivo deste estudo experimental in vitro foi avaliar a Resistência de União ao Cisalhamento (RUC) da colagem de bráquetes em diferentes tratamentos de superfície de resina composta, associados a dois agentes de união. Foram confeccionados 90 corpos de prova com resina composta Filtek Z350 (3M/ESPE). Os corpos de prova foram aleatoriamente divididos em nove grupos (n=10): (1) grupo controle; (2) silano e primer; (3) adesivo universal (5) ácido fosfórico, silano e primer (4) ácido fosfórico, adesivo universal; (6) ácido fluorídrico, silano e primer; (7) ácido fluorídrico e adesivo universal; (8) broca, silano e primer e (9) broca e adesivo universal. Em seguida, foi aplicado na superfície de colagem do bráquete metálico (Standart Edgewise para incisivo central superior ­ Morelli, Sorocaba/SP) a resina ortodôntica Transbond XT/3M. O bráquete foi colado na resina composta Filtek Z350 (3M/ESPE). Após 24 horas foram submetidos ao teste de cisalhamento na máquina Instron, com velocidade de 0,5mm/min. Os dados foram submetidos à ANOVA e teste de Tukey. Em seguida foi avaliado o Índice de Remanescente Adesivo (IRA). Não houve diferença significativa entre os agentes de união quanto à resistência de união (p>0,05). Os oito grupos que receberam agentes de união apresentaram resistência significativamente maior do que o grupo controle (p<0,05). O grupo preparado com ácido fluorídrico apresentou menor resistência que os preparados com ácido fosfórico (com silano + primer transbond) e broca diamantada (com silano + primer transbond e com adesivo universal), p<0,05. Concluiu-se que o tratamento de superfície influencia na resistência de união. (AU)

Abstract The aim of this in vitro experimental study was to evaluate the shear bond strength of bracket bonding in different composite resin surface treatments associated with two bonding agents. Ninety specimens were made with Filtek Z350 composite resin (3M/ESPE). The specimens were randomly divided into nine groups (n=10): (1) control group; (2) silane and primer; (3) universal adhesive (5) phosphoric acid, silane and primer (4) phosphoric acid, universal adhesive; (6) hydrofluoric acid, silane and primer; (7) hydrofluoric acid and universal adhesive; (8) drill, silane and primer and (9) universal drill and adhesive. Transbond XT/3M orthodontic resin was then applied to the bonding surface of the metal bracket (Standart Edgewise for upper central incisor ­ Morelli, Sorocaba/SP). The bracket was bonded to the Filtek Z350 composite resin (3M/ ESPE). After 24 hours they were submitted to the shear bond test on the Instron machine at a speed of 0.5mm/min. Data were submitted to ANOVA and Tukey test. Then the Adhesive Remnant Index (ARI) was evaluated. There was no significant difference between bonding agents regarding bond strength (p> 0.05). The eight groups that received bonding agents showed significantly higher resistance than the control group (p <0.05). The group prepared with hydrofluoric acid presented lower resistance than those prepared with phosphoric acid (with silane + transbond primer) and diamond drill (with silane + transbond primer and universal adhesive), p <0.05. It was concluded that the surface treatment influences the bond strength.(AU)

Temporomandibular disorder symptoms in the university context.

OBJECTIVE: To assess the impact of the university context of different academic fields and class times and the sociodemographic context on the symptoms of temporomandibular disorder (TMD). METHODS: A total of 2912 university students, divided into 26 undergraduate courses distributed in the fields of biology and exact and human sciences in the day and night participated in this study. The symptoms of orofacial pain and TMD were assessed using a self-explanatory questionnaire for screening, as recommended by the American Academy of Orofacial Pain. The data were analyzed using frequency distribution tables and multiple logistic regression at a 5% significance level. RESULTS: There was a significant association between the female sex and the attended class time. Women and undergraduates of night courses were 1.60 times more likely to present symptoms related to TMD compared to women studying during the day. CONCLUSION: It was concluded that, within the university context, women and undergraduate students enrolled in night courses were more likely to present with TMD symptoms.

Orthodontist and periodontist's knowledge, attitudes and aspects of clinical practice, regarding fixed lower orthodontic retainers.

OBJECTIVE: This study aimed to assess the knowledge, attitudes, and aspects of the clinical practice of orthodontists and periodontists, regarding lower fixed orthodontic retainers. METHODS: The orthodontists (n=502) and periodontists (n=269) who participated in this cross-sectional observational study received, via e-mail, questions related to the type of lower fixed retainer, dental biofilm accumulation, oral hygiene, and potential periodontal changes. The data were subjected to chi-square and Fisher's exact tests, at 5% significance level. RESULTS: Both orthodontists (72.3%) and periodontists (58.7%) reported that hygienic retainers accumulate more dental biofilm (p< 0.05), and 64.1% of orthodontists and 58.7% of periodontists considered that modified retainers may lead to periodontal changes (p< 0.05). There was no significant difference between the dental specialties, regarding the type of lower fixed retainer considered the easiest for the patient to perform hygiene (p> 0.05), whereas 48.6% of professionals chose the modified type. CONCLUSION: The modified retainer accumulates a greater amount of dental biofilm and, in the perception of orthodontists and periodontists, it may cause periodontal changes.

Mandibular ramus height and condyle distance asymmetries in individuals with different facial growth patterns: a cone-beam computed tomography study.

PURPOSE: This study aimed to quantify mandibular ramus height and condylar distances asymmetry indexes in adult patients with different vertical facial growth pattern using Cone Beam computed tomography (CBCT). METHODS: An observational cross-sectional study was conducted by using CBCT of 159 patients (mean age 26.36 ± 5.32 years). Vistadent 3D® software was used to determine the facial pattern in 3 groups (N = 53): hypodivergent, normodivergent and hyperdivergent-by Ricketts VERT index. Mandibular ramus height and the condylar linear distance in relation to the median sagittal plane were evaluated. The asymmetry index was calculated considering the right and left sides. Data were analyzed by generalized linear models and Tukey post-hoc test (α = 0.05). RESULTS: Significantly lower values were found for the left and right mandibular ramus height in the hyperdivergent skeletal pattern (P < 0.05). There was no statistically significant difference for condylar and intercondylar distances among the facial groups (P > 0.05). Asymmetry indexes (mandibular ramus height and condylar distance) were similar, and no statistically significant differences were found among the skeletal patterns. In most subjects, the severity of mandibular ramus height asymmetry varied from light to not clinically significant independently of the facial type. CONCLUSION: The findings suggest that vertical facial growth pattern not affected the asymmetry index of mandibular ramus height and the intercondylar distance. The results also demonstrated significantly shorter mandibular ramus height for the hyperdivergent skeletal pattern individuals.

Affective relationships as predictors of TMD symptoms in young adults / Relações afetivas como preditoras de sintomas de DTM em adultos jovens

Abstract Introduction Orofacial pain is a critical TMD symptom that can influence physical and social capacity. Objective To evaluate the association of temporomandibular disorders (TMD) symptoms with affective relationships and demographic variables in young adults. Material and method A cross-sectional study involving 395 young adults was developed. Diagnostic Criteria for TMD, anxiety, and depression were collected from questionnaires. The Dental Health Component of the Index of Orthodontic Treatment Need measures the orthodontic treatment need. Questionnaires also contained questions related to the previous orthodontic treatment. Logistic regression models were adjusted, estimating crude odds ratio with the 95% confidence intervals. The variables with p<0.20 in the analyses were assessed in a multiple logistic regression model, remaining with p≤0.10. Result There was no significant association of TMD symptoms with sex, age, medication use for pain, previous orthodontic treatment, orthodontic treatment need, anxiety, and depression (p>0.05). Individuals without an affective relationship are 1.78 (95%CI: 0.99-3.17) times more likely to report TMD symptoms. Conclusion Affective relationships showed an association with TMD symptoms in young adults.

Resumo Introdução A dor orofacial é um sintoma crítico da DTM que pode influenciar a capacidade física e social. Objetivo Avaliar a associação dos sintomas das desordens têmporomandibulares (DTM) com as relações afetivas e variáveis demográficas em adultos jovens. Material e método Estudo transversal envolvendo 395 adultos jovens foi realizado. Os critérios diagnósticos para DTM, ansiedade e depressão foram coletados a partir de questionários. O Componente de Saúde Bucal do Índice de Necessidade de Tratamento Ortodôntico mensurou a necessidade de tratamento ortodôntico. Os questionários também continham questões relacionadas ao tratamento ortodôntico anterior. Modelos de regressão logística foram ajustados, estimando odds ratio bruto com os intervalos de confiança de 95%. As variáveis com p<0.20 nas análises foram avaliadas em modelo de regressão logística múltipla, permanecendo as variáveis com p≤0.10. Resultado Não houve associação significativa dos sintomas de DTM com sexo, idade, uso de medicamentos para dor, tratamento ortodôntico prévio, necessidade de tratamento ortodôntico, ansiedade e depressão (p>0.05). Indivíduos sem relacionamento afetivo têm 1.78 (IC95%:0.99-3.17) vezes mais chance de relatar sintomas de DTM. Conclusão Os relacionamentos afetivos mostraram associação com os sintomas de DTM em adultos jovens.

How is orthodontic treatment need associated with perceived esthetic impact of malocclusion in adolescents?

INTRODUCTION: The purpose of this research was to evaluate the esthetic impact of orthodontic treatment need in adolescents. METHODS: This cross-sectional study was performed with 1172 adolescents aged 12 (n = 638) years and 15 (n = 534) years from Salvador, Bahia, Brazil. The esthetic impact was the following outcome variables: Aesthetic Component of the Index of Orthodontic Treatment Need and the Oral Aesthetic Subjective Impact Scale. The normative orthodontic treatment need was assessed by the Dental Health Component of the Index of Orthodontic Treatment Need (IOTN-DHC) and by the Dental Aesthetic Index (DAI). Logistic regression models were adjusted, estimating crude odds ratio with the respective 95% confidence intervals. The variables with P ≤0.20 in the individual analyses were tested in multiple logistic regression models, remaining the variables with P ≤0.05. RESULTS: The results showed a statistically significant association for the esthetic impact (Aesthetic Component of the Index of Orthodontic Treatment Need and the Oral Aesthetic Subjective Impact Scale) when assessing the normative orthodontic treatment need by the DAI (P <0.05). However, there was no significant association between subjective esthetic impact and normative needs when using the IOTN-DHC (P >0.05). CONCLUSIONS: DAI was more associated with the adolescents' esthetic concern than IOTN-DHC and should be the Index of Orthodontic Treatment Need to be selected to complement the patient's self-perception.

Immediate and late follow-up effects of class III early correction in cleft lip and palate patients treated with maxillary protraction / Efeitos imediatos e a longo prazo da correção precoce da classe III em pacientes com fissura lábio palatal tratados com protração maxilar

Abstract Introduction There is no consensus about the immediate and latte follow-up effects of maxillary protraction in cleft lip and palate patients. Objective To evaluate the stability of Class III early treatment in cleft lip and palate patients through maxillary expansion and protraction. Material and method The sample consists in three lateral cephalometric radiographs of 28 patients (mean pre-treatment age of 6.7±1.8 years) who presented cleft lip and palate and were treated with maxillary expansion and Petit facial mask. The angular (SNA, SNB, ANB, SN.GoGn, FMA, Z Angle) and linear (overjet, Co-A, Co-Gn, Nperp-A, Nperp-Pg, AO-BO) cephalometric measures were evaluated through the Dolphin® software, in three moments: T0 (initial), T1 (after treatment), and T2 (follow-up). Data were submitted to the analysis of variance (ANOVA) and Tukey-Kramer test. The correlation between cephalometric measures and patient's age was determined by Pearson's chi-squared test. Result The SNA, ANB, and AO-BO measures increased considerably (p<0.05), and they did not present any differences compared to the initial ones after the follow-up time. The overjet measure increased (p<0.05) after treatment and, even with its decrease at the follow-up time, it was still higher than at the beginning (p<0.05). The Z angle showed improvement with treatment and remained stable at the follow-up time. Conclusion After treatment (maxillary expansion associated with protraction), there was a skeletal pattern improvement. During the follow-up period, those alterations decreased to a measure close to the beginning. There was an improvement in the dental pattern and facial profile that continued in the follow-up period.

Resumo Introdução Não há consenso sobre os efeitos imediatos e tardios da protração maxilar em pacientes com fissura lábio palatal. Objetivo avaliar a estabilidade do tratamento precoce da Classe III em pacientes com fissura labiopalatina por meio da expansão e protração maxilar. Material e método A amostra consistiu de 28 pacientes com fissura lábio palatal com (média de idade pré tratamento de 6.7±1.8) com fissura lábio palatal transforame unilateral, tratados com disjuntor maxilar e com máscara facial de Petit. Por meio de análise em telerradiografias laterais as grandezas angulares (SNA, SNB, ANB, SN.GoGn, FMA, Ângulo Z) e lineares (overjet, Co-A, Co-Gn, Nperp-A, Nperp-Pg, AO-BO) foram avaliadas, com o software Dolphin®, nos tempos: inicial (T0), após o tratamento (T1) e acompanhamento de 2 a 6 anos (T2). Os dados foram submetidos à ANOVA e teste Tukey-Kramer. A correlação entre as grandezas cefalométricas e a idade do paciente foi determinada pelo teste de Pearson. Todas as análises foram realizadas com nível de significância de 5%. Resultado SNA, ANB e AO-BO aumentaram significativamente (p<0,05) e após o período de acompanhamento voltaram a ser semelhantes às iniciais (p>0,05). O overjet aumentou significativamente após o tratamento e mesmo tendo diminuído com o tempo, ainda se apresentava maior quando comparado ao momento inicial (p<0,05). O ângulo Z apresentou melhora com o tratamento e se manteve estável no período de acompanhamento (p<0,05). Conclusão após o tratamento (disjunção associada à protração maxilar) houve melhora do padrão esquelético. No período de acompanhamento, verificou-se que essas melhoras voltaram a medidas próximas das iniciais. Houve melhora no padrão dentário e no perfil facial que se manteve no período de controle.

Comorbidity of TMD and malocclusion: impacts on quality of life, masticatory capacity and emotional features

Aim: to evaluate the synergic impact of muscular TMD and malocclusion on quality of life, masticatory capacity and emotional features of young adults. Methods: this cross-sectional study comprised 4 groups (n= 15): G1, individuals without TMD or malocclusion; G2, with TMD and malocclusion; G3, with TMD and without malocclusion, and G4, without TMD and with malocclusion. Muscular TMD was diagnosed by RCD/TMD. Data included quality of life (OHIP-14), masticatory capacity test (X50), emotional stress (PSS-14), depression (MDI), pain intensity and salivary cortisol. Comparative statistical analysis included One-way ANOVA and Tukey post hoc test (X50, stress and cortisol) and GENMOD followed by Wald test (OHIP-14 and pain data). Fisher's and Pearson's association analysis were carried out. Results: Comorbidity of muscular TMD and malocclusion leads to significant lower masticatory capacity (p<0.05). TMD groups independently of the occlusal condition had considerably lower OHIP-14 scores and higher stress levels (self-perceived and hormonal) (p<0.05). There was no statistically significant difference of emotional depression among groups. A significant positive correlation was observed among quality of life, stress and pain perception. Conclusion: muscular TMD in the overlap of malocclusion potentializes their negative effect on masticatory capacity. In addition, the hindering effect of the comorbidity is variable, however, TMD has a greater negative impact on quality of life and stress, whilst malocclusion on mastication

Evaluation of friction in metal, ceramic and self-ligating brackets submitted to sliding mechanics / Avaliação do atrito em braquetes metálicos, cerâmicos e autoligados submetidos à mecânica de deslizamento

Objective: With the purpose of evaluating the best results for a proposed orthodontic treatment, the aim of this research was to verify the frictional force between archwires and orthodontic brackets in sliding mechanics. Material and method: Three different types of brackets were used: conventional metal, self-ligating metal and conventional polycrystalline esthetic type (n=10), totaling 30 brackets and .019 x .025 inches stainless steel rectangular wire. An acrylic plate was used. The wire was fixed to the brackets (conventional metal and conventional polycrystalline esthetic) with esthetic elastomeric ligation. The acrylic plate together with the 019 x 0.025 inches wire fitted to it was attached to the base plate of the Instron 4411 universal test machine so that it was positioned perpendicular to the ground. The bracket was drawn through the archwire segment at a speed of 5 mm / minute for a distance of 5 mm. For each type of bracket, ten tests were performed, with the bracket and archwire set being changed for each repetition. Data were analyzed by one-way Analysis of Variance (p <0.0001) and the Tukey Test (p <0.05). Result: Convencional metal and self-ligating brackets presented similar friction (p>0.05), while ceramic bracket presented higher frictional resistance during sliding test (p<0.05). Conclusion: It was concluded that the type of bracket influenced the frictional force value obtained, and better results were found when the metal and self-ligating brackets were used. The ceramic bracket presented a higher frictional force value.

Objetivo: Visando avaliar os melhores resultados para um tratamento ortodôntico de premissa, esta pesquisa teve como objetivo verificar a força de atrito entre fio e braquetes ortodônticos na mecânica de deslizamento. Material e método: Foram utilizados três diferentes tipos de braquetes, sendo metálico convencional, metálico autoligado e estético policristalino convencional (n=10), totalizando 30 braquetes e fio retangular de aço inoxidável .019 x .025 polegadas. Foi utilizada uma placa de acrílico. O fio foi fixado aos braquetes (metálico convencional e estético policristalino convencional) com ligadura elastomérica estética. A placa de acrílico juntamente com o fio .019 x .025 polegadas montado foi fixada ao mordente da base da máquina de ensaio universal Instron 4411, de maneira que ficou posicionada perpendicular em relação ao solo. O braquete foi tracionado à velocidade de 5 mm/minuto através do segmento do fio por uma distância de 5mm. Para cada tipo de braquete foi realizado dez testes havendo a troca do conjunto braquete e fio em cada repetição. Os dados foram analisados por Análise de Variância um fator (p<0,0001) e Teste de Tukey (p<0,05). Resultado: Braquetes metálicos convencionais e autoligáveis apresentaram atrito similar (p>0,05), enquanto braquetes cerâmicos apresentaram maior resistência ao atrito durante o teste de deslizamento (p<0,05). Conclusão: Conclui-se que o tipo de braquete influenciou no valor de atrito obtido, sendo que melhores resultados foram encontrados quando foram utilizados os braquetes metálicos e autoligados. O braquete cerâmico apresentou maior atrito.

Plasma proteins in the acquired denture pellicle enhance substrate surface free energy and Candida albicans phospholipase and proteinase activities.

AIM: The objective of the present study was to determine if blood plasma proteins could change the proteome of the acquired denture pellicle by label-free quantitative proteomics. As pellicle proteome modulates the interaction between substrates and Candida cells, we investigated its effect on the surface free energy (SFE) of the coated resin and on Candida albicans phospholipase and aspartyl proteinase activities. METHODS: Poly(methylmethacrylate) discs were exposed to saliva (control) or saliva enriched with blood plasma (experimental group). The pellicle proteome was analyzed by mass spectrometry coupled with liquid chromatography. SFE was determined by acid-base technique. After biofilm formation, phospholipase and proteinase activities were determined accordingly to classic plate methods. Data were analyzed by two-way anova and Tukey test (P < 0.05). RESULTS: α-Amylase, cystatins, mucins, and host-immune system proteins were the main proteins identified in the control group. Fibrinogen and albumin were observed only in the experimental group. Coated discs of the experimental group presented an increased SFE (P < 0.05). For both enzymes tested, the experimental group showed higher proteolytic activity (P < 0.001). CONCLUSION: Blood plasma changes the proteome of the acquired denture pellicle, increasing surface free energy and the activity of Candida albicans phospholipase and aspartyl proteinase.

Acquired pellicle as a modulator for dental erosion.

Dental erosion is a multifactorial condition that can result in the loss of tooth structure and function, potentially increasing tooth sensitivity. The exposure of enamel to acids from non-bacterial sources is responsible for the progression of erosion. These erosive challenges are counteracted by the anti-erosive properties of the acquired pellicle (AP), an integument formed in vivo as a result of selective adsorption of salivary proteins on the tooth surface, containing also lipids and glycoproteins. This review provides an in-depth discussion regarding how the physical structure of the AP, along with its composition, contributes to AP anti-erosive properties. The physical properties that contribute to AP protective nature include pellicle thickness, maturation time, and site of development. The pellicle contains salivary proteins embedded within its structure that demonstrate anti-erosive properties; however, rather than individual proteins, protein-protein interactions play a fundamental role in the protective nature of the AP. In addition, dietary and synthetic proteins can modify the pellicle, enhancing its protective efficiency against dental erosion. The salivary composition of the AP and its corresponding protein-profile may be employed as a diagnostic tool, since it likely contains salivary biomarkers for oral diseases that initiate at the enamel surface, including dental erosion. Finally, by modifying the composition and structure of the AP, this protein integument has the potential to be used as a target-specific treatment option for oral diseases related to tooth demineralization.

Proteomic evaluation of acquired enamel pellicle during in vivo formation.

Acquired enamel pellicle (AEP) is a protein film that forms on the enamel surface of teeth by selective adsorption of proteins and peptides present in the mouth. This protein film forms the interface between enamel and the damage oral biofilm, which modulates the attachment of bacteria found in oral biofilm. The overall goal of this study was to gain insight into the biological formation of the human in vivo AEP. This study hypothesized that AEP is created by the formation of successive protein layers, which consist of initial binding to enamel and subsequent protein-protein interactions. This hypothesis was examined by observing quantitative and qualitative changes in pellicle composition during the first two hours of AEP formation in the oral cavity. Quantitative mass spectrometry approaches were used to generate an AEP protein profile for each time-point studied. Relative proteomic quantification was carried out for the 50 proteins observed in all four time-points. Notably, the abundance of important salivary proteins, such as histatin 1, decrease with increasing of the AEP formation, while other essential proteins such as statherin showed constant relative abundance in all time-points. In summary, this is the first study that investigates the dynamic process to the AEP formation by using proteomic approaches. Our data demonstrated that there are significant qualitative and quantitative proteome changes during the AEP formation, which in turn will likely impact the development of oral biofilms.

Proteome analysis of the plasma protein layer adsorbed to a rough titanium surface.

In this study a label-free proteomic approach was used to investigate the composition of the layer of protein adsorbed to rough titanium (Ti) after exposure to human blood plasma. The influence of the protein layer on the surface free energy (SFE) of the Ti was evaluated by contact angle measurements. Ti discs were incubated with blood plasma for 180 min at 37 °C, and the proteins recovered were subjected to liquid chromatography coupled to tandem mass spectrometry analysis. A total of 129 different peptides were identified and assigned to 25 distinct plasma proteins. The most abundant proteins were fibronectin, serum albumin, apolipoprotein A-I, and fibrinogen, comprising 74.54% of the total spectral counts. Moreover, the protein layer increased the SFE of the Ti (p < 0.05). The layer adsorbed to the rough Ti surface was composed mainly of proteins related to cell adhesion, molecule transportation, and coagulation processes, creating a polar and hydrophilic interface for subsequent interactions with host cells.

Proteome and peptidome of human acquired enamel pellicle on deciduous teeth.

Understanding the composition and structure of the acquired enamel pellicle (AEP) has been a major goal in oral biology. Our lab has conducted studies on the composition of AEP formed on permanent enamel. The exhaustive exploration has provided a comprehensive identification of more than 100 proteins from AEP formed on permanent enamel. The AEP formed on deciduous enamel has not been subjected to the same biochemical characterization scrutiny as that of permanent enamel, despite the fact that deciduous enamel is structurally different from permanent enamel. We hypothesized that the AEP proteome and peptidome formed on deciduous enamel may also be composed of unique proteins, some of which may not be common with AEP of permanent enamel explored previously. Pellicle material was collected from 10 children (aged 18-54 months) and subjected to mass spectrometry analysis. A total of 76 pellicle proteins were identified from the deciduous pellicle proteome. In addition, 38 natural occurring AEP peptides were identified from 10 proteins, suggesting that primary AEP proteome/peptidome presents a unique proteome composition. This is the first study to provide a comprehensive investigation of in vivo AEP formed on deciduous enamel.